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| Avellaneda-Torres,Lizeth Manuela; Pulido,Claudia Patricia Guevara; Rojas,Esperanza Torres. |
| A systematized survey was conducted to find soil-borne microbes that degrade cellulose in soils from unique ecosystems, such as the Superpáramo, Páramo, and the High Andean Forest in the Nevados National Natural Park (NNNP), Colombia. These high mountain ecosystems represent extreme environments, such as high levels of solar radiation, low atmospheric pressure, and extreme daily changes in temperature. Cellulolytic activity of the microorganisms was evaluated using qualitative tests, such as growth in selective media followed by staining with congo red and iodine, and quantitative tests to determine the activity of endoglucanase, β-glucosidase, exoglucanase, and total cellulase. Microorganisms were identified using molecular markers, such as the 16S rRNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellulase; Endoglucanase; Β-glucosidase; Exoglucanase; Páramo; High Andean Forest. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400011 |
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| Gottschalk,Leda Maria Fortes; Paredes,Raquel de Sousa; Teixeira,Ricardo Sposina Sobral; Silva,Ayla Sant'Ana da; Bon,Elba Pinto da Silva. |
| The production of xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by Aspergillus awamori 2B.361 U2/1, a hyper producer of glucoamylase and pectinase, was evaluated using selected conditions regarding nitrogen nutrition. Submerged cultivations were carried out at 30 ºC and 200 rpm in growth media containing 30 g wheat bran/L as main carbon source and either yeast extract, ammonium sulfate, sodium nitrate or urea, as nitrogen sources; in all cases it was used a fixed molar carbon to molar nitrogen concentration of 10.3. The use of poor nitrogen sources favored the accumulation of xylanase, β-xylosidase and ferulic acid esterase to a peak concentrations of 44,880; 640 and 118 U/L, respectively, for sodium nitrate and of 34,580, 685 and 170... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus awamori 2B.361 U2/1; Cellulases; Hemicellulases; Β-glucosidase; Nitrogen nutrition. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200037 |
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| Duarte,Silvana; Paiva,Marco A. R.; Lara,Camila C.; Bemquerer,Marcelo P.; Araújo,Francisco Gerson. |
| The enzymatic activities involved in the digestion of proteins and carbohydrates were compared among three organs of the digestive track of two Siluriformes fish species, and between two areas: a reservoir, and an area downriver of it. Our aim was to test the hypothesis that the digestive organs of species with varied feeding habits have different enzymatic activities, and that the enzymatic activity differs among seasons and environmental conditions. The iliophagous/herbivorous species Hypostomus auroguttatus Kner, 1854 had higher trypsin-like, chymotrypsin-like peptidase and β-glucosidase activity in the intestine when compared with the omnivorous species Pimelodus maculatus Lacepède, 1803, whereas the latter had more hepatic trypsin-like activity than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic activity; Fishes; Physiology; Reservoirs; Siluriformes; Peptidases; Β-glucosidase. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702013000300006 |
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| Silva,Andréa Scaramal da; Colozzi Filho,Arnaldo; Nakatani,André Shigueyoshi; Alves,Sérgio José; Andrade,Diva de Souza; Guimarães,Maria de Fátima. |
| Integrated crop-livestock systems (ICLs) are a viable strategy for the recovery and maintenance of soil characteristics. In the present study, an ICL experiment was conducted by the Instituto Agronômico do Paraná in the municipality of Xambre, Parana (PR), Brazil, to evaluate the effects of various grazing intensities. The objective of the present study was to quantify the levels of microbial biomass carbon (MBC) and soil enzymatic activity in an ICL of soybean (summer) and Brachiaria ruziziensis (winter), with B. ruziziensis subjected to various grazing intensities. Treatments consisted of varying pasture heights and grazing intensities (GI): 10, 20, 30, and 40 cm (GI-10, GI-20, GI-30, and GI-40, respectively) and a no grazing (NG) control. The microbial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial carbon; Microbial respiration; Acid phosphatase; Β-glucosidase; Arylsuphatase; Fluorescein diacetate hydrolysis; Carbono microbiano; Respiração microbiana; Fosfatase ácida; Β-glucosidase; Arilsulfatase; Hidrólise do diacetato de fluoresceína. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000100040 |
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| Santos,Bruna Silveira Lamanes dos; Gomes,Arthur Filipe Sousa; Franciscon,Emanuele Giuliane; Oliveira,Jean Maikon de; Baffi,Milla Alves. |
| Nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at Brazilian Cerrado and identified up to species level on the basis of the gene sequencing of 5.8S-ITS and 26S ribosomal DNA regions. Four species were identified: Kluyveromyces marxianus, Aspergillus niger, Aspergillus sydowii and Aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. Among them, three strains were selected as good producers of hemicellulolitic enzymes: A. niger (SBCM3), A. sydowii (SBCM7) and A. fumigatus (SBC4). The best β-xylosidase producer was A. niger SBCM3 strain. This crude enzyme presented optimal activity at pH 3.5 and 55 °C (141... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-glucosidase; Β-xylosidase; Xylanase; Aspergillus; Sugarcane bagasse. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300903 |
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